FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314399

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314399
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4652758
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG7750.033.09540648-49
CTCGTAT7900.033.0671944-45
CGTATGC8700.030.02652746-47
TATCTCG8000.027.90254640-41
TCGTATG9200.027.87838644-45
TCTCGTA8100.027.5589642-43
GATCGGA18550.027.4550341
ATCTCGT9100.025.8353242-43
ATCGGAA20250.025.6031682
ATGCCGT9600.025.23333448-49
TGCCGTC9150.023.61962350-51
TCGGAAG21750.023.5832083
CGGAAGA22750.022.9646034
GCCGTCT10800.019.57126650-51
CGTCTTC10900.018.0843152-53
CCGTCTT11200.017.59990952-53
CGTCTGA15200.017.02963316-17
ACACGTC15800.016.9836912-13
GTATGCC16850.015.64425146-47
ACGTCTG18100.014.69410414-15
Produced by FastQC (version 0.11.5)