FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314401

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314401
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5229594
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3550.020.06861748-49
TCGTATC4600.015.48736638-39
CGTATGC4850.014.68968146-47
CTCGTAT4800.014.34807944-45
GTCACGA5850.013.80541128-29
TCTCGTA4950.013.43332942-43
CGTTGGA17100.012.8003731
GATCGGA11200.012.7457131
TCGTATG5850.012.1787444-45
ATCGGAA11350.012.1529032
ATGCCGT6050.011.775848-49
TATCTCG5700.011.665338540-41
TCGGAAG12900.011.4131563
ATTCGTA6700.010.98750136-37
CGGAAGA14200.010.7034664
AAACGGG21500.010.3856677
ACGAATT7400.010.26890432-33
AACGGGA17600.010.2577018
ATCTCGT6750.010.20293242-43
CGAATTC7800.010.04674132-33
Produced by FastQC (version 0.11.5)