FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314403

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314403
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4680155
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3750.024.06509248-49
CTCGTAT4550.020.87697644-45
TCTCGTA4900.018.41627742-43
TATCTCG5250.017.18834140-41
GATCGGA10850.016.6698531
CGTATGC5900.016.1000446-47
ATCGGAA12450.014.9002752
TCGTATG6400.014.84222344-45
TCGGAAG13750.014.1620293
ATGCCGT6850.013.86770648-49
CGGAAGA13700.013.8676334
AATTCGC2100.00729103513.5729347
TGCCGTC7050.012.80078850-51
ATCTCGT7150.012.62094542-43
GCCGTCT7750.011.64458750-51
CGTTGGA18600.010.2358751
CGTCTGA9650.010.08963616-17
CGTCTTC9000.09.76319852-53
CCGTCTT8850.09.39199152-53
ACACGTC10450.09.08987712-13
Produced by FastQC (version 0.11.5)