FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314406

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314406
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5039148
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA7050.039.74202358-59
TCGCCGT7800.035.310856-57
GTCGCCG8100.034.29612456-57
GCCGTAT8550.033.32515758-59
CGTATCA9250.031.06010260-61
GGTCGCC9200.030.96897354-55
TGGTCGC9150.030.35974754-55
CCGTATC9550.029.58712860-61
GTGGTCG9700.028.88323252-53
GAGCGTC20100.026.466169
TCTCGGT11900.023.54955148-49
AGAGCGT22400.023.5361428
TCGGTGG13350.021.16805550-51
AAGAGCG27100.019.9790277
GATCGGA28600.019.405221
CGGAAGA28950.019.3583324
CTCGGTG14800.019.25604648-49
TCGGAAG28150.019.2332343
ATCGGAA28150.019.0635762
CGGTGGT15100.019.02933950-51
Produced by FastQC (version 0.11.5)