FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314408

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314408
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6022548
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGTAGATCT97510.16190821559247018Illumina Single End PCR Primer 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA12650.042.23249458-59
GAGCGTC26400.040.8407069
TCGCCGT13350.040.0165656-57
GTCGCCG13550.038.9002356-57
AGAGCGT27900.038.1326568
TGGTCGC15200.034.9880354-55
GGTCGCC15700.034.93231654-55
GCCGTAT15800.034.71439758-59
GTGGTCG15750.034.22015852-53
GTACGTC15650.033.9918630-31
CGTATCA16100.033.77314860-61
GATCGGA33200.033.137871
AAGAGCG32400.033.127167
CCGTATC16450.032.6215460-61
ATCGGAA33200.032.4719662
GTGTACG17050.032.0300928-29
TCGGAAG34050.031.8003063
TACGTCC16500.031.38108432-33
CGGAAGA35100.030.9843184
TCTCGGT19350.028.72124348-49
Produced by FastQC (version 0.11.5)