FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314409

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314409
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6479350
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG8550.025.82935148-49
CTCGTAT9150.023.35678544-45
CGTATGC9700.023.25636746-47
TCTCGTA9300.023.23602342-43
ATCGGAA19100.022.6602782
GATCGGA18600.022.6004661
TCGGAAG19250.022.4576723
ATGCCGT11100.020.96523748-49
GCCGTCT11100.020.53721850-51
TCGTATG11250.020.47438444-45
TATCTCG11200.019.71862840-41
CGGAAGA21750.019.6539654
TGCCGTC12200.018.29621950-51
CGTTGGA22750.017.4278741
ATCTCGT11950.017.0896842-43
TCCGATC4507.2759576E-1216.9845921
CCGTCTT14200.016.55567652-53
CGTCTTC14450.016.1049152-53
CCGATCT5450.015.70844652
ACACGTC15600.014.91653512-13
Produced by FastQC (version 0.11.5)