FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314410

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314410
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6479350
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA6050.033.91734358-59
TCGCCGT7100.030.54293856-57
GTCGCCG6750.030.39940856-57
GCCGTAT7250.029.58996258-59
GAGCGTC15100.026.86859
TGGTCGC7900.025.97447654-55
GGTCGCC8750.025.31668354-55
CGGCGTA1151.4533683E-424.3305078
GTGGTCG9400.023.07117852-53
AGAGCGT18400.022.5564088
CCGTATC9250.022.43569460-61
CGTATCA9800.022.36624160-61
GATCGGA18250.021.8105981
ATCGGAA20150.020.6004352
CGGAAGA21700.019.7705674
AAGAGCG20800.019.7295487
TCGGAAG20950.019.365543
GCGTCGT10900.019.03858410-11
CGTCGTG10950.018.9512112-13
AGCGTCG11200.018.32043510-11
Produced by FastQC (version 0.11.5)