FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314413

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314413
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5088220
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4400.018.88929748-49
TAATGCG5200.016.44258134-35
GCGCATC4550.016.1797638-39
AATGCGC5850.015.83359834-35
GATCGGA10100.015.6032611
ATCGGAA10800.015.4120292
TCGGAAG11450.015.3505813
CTCGTAT6050.014.5226844-45
CGTTGGA21200.014.4167351
ATGCCGT5850.014.207334548-49
CGTATGC6150.013.90014446-47
TCTCGTA5700.013.33178742-43
TCGTATG6900.013.07780844-45
ATGCGCA6850.012.827105536-37
CGCATCT7050.012.12649238-39
CGGAAGA13900.011.9591624
CGTAGGT3206.738764E-411.9388591
TGCCGTC7000.011.87315750-51
AAACGGG24200.011.3818597
TGCGCAT7150.011.29250736-37
Produced by FastQC (version 0.11.5)