FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314416

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314416
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4475313
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA1900.033.138458-59
GTCGCCG2200.027.55954756-57
TCGCCGT2300.027.37520256-57
ACGACGC1100.003202562221.197645
GAGCGTC6050.020.8126939
GCGTCGT3050.020.64186510-11
CACGACG1405.494776E-419.9865674
GATCGGA6650.019.9477391
GCCGTAT3050.019.11443958-59
GGTCGCC3250.018.6561154-55
CGTCGTG3500.018.65433912-13
TGGTCGC3500.017.3235354-55
ATCGGAA7550.017.2967642
AGAGCGT7850.016.6342568
AGCGTCG3800.016.56781410-11
TCGGAAG8050.016.2209823
AGTACGT4650.015.99155636-37
GAAACGG19250.015.9892546
CGTTGGA17500.015.9724391
ACGGGAT12300.015.9244369
Produced by FastQC (version 0.11.5)