FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314417

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314417
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7369674
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT103340.14022329888676216TruSeq Adapter, Index 3 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA36100.035.305081
TATGCCG18100.035.0293548-49
ATCGGAA36750.034.813882
AATGCGC18450.034.49857334-35
TCGGAAG37350.034.2139363
GCGCATC18000.032.85064338-39
CGTATGC19300.032.7283246-47
TAATGCG19600.032.47442234-35
CGGAAGA39800.032.4612544
ATGCGCA19850.032.0606436-37
TCTCGTA18400.032.00700442-43
CTCGTAT19650.031.78283144-45
TGCGCAT19250.030.96284536-37
TCGTATG20850.030.75084744-45
TGCCGTC20550.030.39067550-51
GCCGTCT21000.029.96560150-51
ATGCCGT21400.029.62762848-49
CGCATCT21100.027.911738-39
ATCTCGT21500.027.3920442-43
CGTCTTC23700.026.35212552-53
Produced by FastQC (version 0.11.5)