FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314418

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314418
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7369674
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCT81710.11087328964619059Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTC27100.042.8531889
CGCCGTA14150.041.20047458-59
GTCGCCG14950.039.46277656-57
AGAGCGT30550.038.7758068
TCGCCGT15400.037.70395756-57
TGGTCGC17200.034.70757754-55
GATCGGA34850.034.5391241
GTGGTCG17950.034.03975752-53
GGTCGCC17650.033.954854-55
TCGGAAG35650.033.7512323
GCCGTAT17600.033.65423658-59
ATCGGAA36200.033.3727042
AAGAGCG36450.032.883197
CGTATCA18050.032.55759460-61
CGGAAGA37650.032.082214
CCGTATC18750.031.09336760-61
TCGGTGG21750.028.09324850-51
TCTCGGT22000.027.56339548-49
GCGTCGT21400.027.46049710-11
CGGTGGT22750.026.85838150-51
Produced by FastQC (version 0.11.5)