FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314419

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314419
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5409727
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA21650.015.6576191
AAACGGG24600.013.7067447
TATGCCG3301.8189894E-1213.6717748-49
AACGGGA19750.013.4657958
ACGGGAT16050.013.0193349
GCGCATC4100.012.16277238-39
GAAACGG27900.012.0855166
GGAAACG27250.011.1538445
TAATGCG4352.1282176E-1010.37293434-35
ACGGGTA4205.928424E-410.1766389
CGTATGC4901.8189894E-109.69212546-47
CTCGTAT4453.4706318E-99.60509344-45
ATGCGCA5451.4551915E-119.58564436-37
TGCGCAT5308.185452E-119.40889536-37
AACGGGT6655.6861227E-69.283958
AATGCGC5451.3569661E-98.71506834-35
TCTCGTA5451.250919E-88.27854842-43
CGCATCT6505.293259E-108.03723138-39
TCGTATG5358.029565E-87.98928444-45
CCCAAAG119200.07.97080371
Produced by FastQC (version 0.11.5)