FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314421

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314421
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6177294
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC4150.021.8629841
GATCGGA9400.021.8446241
ATCGGAA10950.019.9823822
CCGATCT5850.017.8883532
TATGCCG5600.017.80923548-49
CTCGTAT6050.016.87720744-45
CGTATGC6200.016.85161246-47
TCGGAAG13150.015.8961543
TCGTATG7100.014.38128244-45
CGTTGGA22250.013.9504211
TATCTCG6850.013.51974640-41
TCTCGTA7650.012.72673442-43
AAACGGG25200.012.4378937
CGGAAGA16450.012.4160474
AACGGGA20500.011.814628
ATGCCGT8650.011.804193548-49
GCCGTCT8250.011.51313450-51
TGCCGTC8700.011.46350850-51
GAAACGG27900.011.4044426
ATCTCGT8550.011.10934442-43
Produced by FastQC (version 0.11.5)