FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314430

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314430
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5527242
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA7150.039.517358-59
GAGCGTC14650.037.9358949
CGGACGA1650.037.4171874
GATCGGA16550.037.0306471
TCGCCGT8350.033.55320456-57
ATCGGAA17050.033.4250832
GTCGCCG8650.032.93848456-57
TCGGACG2200.032.3799633
AGAGCGT16850.032.1342168
GCCGTAT9900.030.45894458-59
GTACGTC8800.030.22731830-31
CGGAAGA19000.029.9943434
AAGAGCG19750.028.856367
CCGTATC10250.028.7239560-61
TCGGAAG19850.028.709693
TGGTCGC10100.027.97372454-55
GGTCGCC10250.027.56435654-55
GTGTACG10750.027.38869728-29
CGGCATA3300.027.3465448
TACGTCC9550.027.10969732-33
Produced by FastQC (version 0.11.5)