FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314431

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314431
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4819681
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA18750.016.5531221
AACGGGA18250.014.5728268
AAACGGG21600.014.0716457
ACGGGAT14050.013.8588189
GAAACGG22700.013.5989746
TATGCCG3109.094947E-1113.0223548-49
GGAAACG23350.012.203465
CCCCGTA3100.007215231710.782121
CGGCTAT3355.11518E-810.6337634-35
CGTATGC3802.6684575E-910.62349746-47
CTCGTAT3152.5101508E-710.55395144-45
GATCGGA7451.3460522E-109.613971
ACCGGCT4606.1190804E-99.29341732-33
CCCGAAA4300.0078441138.8836071
TCGTATG4058.0951577E-78.7949644-45
GTTGGAA46450.08.7034632
ATCGGAA8853.83352E-88.5987552
TCGGAAG10607.550625E-98.5155083
CGATAAT5709.676614E-48.3770861
TCTCGTA4605.6563294E-78.25978742-43
Produced by FastQC (version 0.11.5)