FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314432

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314432
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4819681
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA1250.026.11802358-59
GTCGCCG2201.2732926E-1015.89985256-57
TCGCCGT2404.7111826E-1014.57486456-57
CGTTGGA16550.014.3153071
GGTCGCC3007.094059E-1113.21495554-55
GAGCGTC6101.2860255E-912.2328849
GTCGGAA2750.003517578811.8730592
GCCGTAT3405.711627E-1011.65983158-59
CGGCATA2800.00402972711.6591058
TGGTCGC3457.2759576E-1011.49126654-55
GCGTCGT3152.7064743E-811.1041410-11
ACGGGAT11850.011.019929
CCCAACG4003.860219E-410.66132451
CGTCGTG3408.307143E-810.28771312-13
GTGGTCG3652.1378582E-810.22284852-53
AACGGGA17200.010.0322538
AAACGGG21850.010.0316337
GGAAACG23500.09.5256395
GAAACGG23150.09.4682046
AGCGTCG3855.066977E-79.08520510-11
Produced by FastQC (version 0.11.5)