FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314434

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314434
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4309999
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA1401.9845174E-919.98769458-59
TCGCCGT2151.5625119E-915.18480356-57
GTCGCCG2101.8728315E-814.43589256-57
CGTTGGA17350.012.5596631
CCCGAAA4701.2477354E-511.087011
AAACGGG20000.010.9594117
ACGGGAT11900.010.5814759
GATCGGA5405.0207927E-610.5270611
CCACGTA4952.2140244E-510.52706051
CCGAAAT4551.09468565E-410.4113791
AACGGGA15800.010.3305938
ATCGGAA6808.285497E-810.2890972
GCCGTAT2951.5560599E-610.2761658-59
GAGCGTC5154.06868E-59.9612749
GAAACGG21450.09.7837316
CCGTATC2901.4162597E-59.64889660-61
GATTTCG9752.3283064E-109.5659835
GGAAACG21900.09.3694225
GGTCGCC3409.489888E-68.91644254-55
AACGGGT5255.400834E-48.8829048
Produced by FastQC (version 0.11.5)