FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314436

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314436
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5158981
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA2550.029.2620858-59
TCGCCGT3000.026.42744356-57
ACGACGC1652.8516715E-622.61272
GTCGCCG3600.021.37513756-57
GCCGTAT4250.019.75190258-59
CGACGCT2302.107552E-618.5432821
TGGTCGC5100.016.460454-55
CGTTGGA21200.015.8706391
CACGACG2406.278922E-515.7961281
GAGCGTC9650.014.9809279
GTGGTCG5750.014.60029352-53
GGTCGCC5550.014.2854554-55
AACGGGA19150.013.6370168
CCGTATC5850.013.55246860-61
AAACGGG24650.013.2429717
AGAGCGT11150.012.965438
CGTATCA6350.012.48534560-61
GATCGGA12600.012.4112441
GAAACGG27500.012.2096766
GGAAACG26500.011.9662745
Produced by FastQC (version 0.11.5)