FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314437

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314437
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3594748
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT46660.1298004755827112TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA12750.045.543981
ATCGGAA13850.041.2112922
TCGGAAG15050.037.8678443
TATGCCG7250.037.01429448-49
CGGAAGA15450.036.887964
CTCGTAT7950.033.1575144-45
TCTCGTA8200.031.27734642-43
CGTATGC8700.031.11799846-47
TCGTATG9000.030.34459744-45
ATGCCGT9300.029.36594248-49
ATCTCGT9050.028.0772942-43
CCGGAGA10500.027.36553634-35
TGCCGTC8600.027.06311650-51
GCCGTCT8750.026.87059850-51
CACTCCG11000.025.69685730-31
CGGAGAA11600.024.77052936-37
ACTCCGG11550.024.67181632-33
TCCGGAG12100.023.74695234-35
CGTCTGA12050.023.6480916-17
ACACGTC12200.023.55198112-13
Produced by FastQC (version 0.11.5)