FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314438

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314438
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3594748
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAGATCT40950.11391619106540987Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA11050.046.2889251
GAGCGTC11100.044.5025759
CGCCGTA5900.041.45199258-59
ATCGGAA11700.040.9956472
TCGGAAG12400.040.5979963
AGAGCGT12100.040.4293178
TCGCCGT6200.039.44460356-57
GTCGCCG6650.037.84654656-57
CGGCATA1551.8189894E-1236.7700428
GCCGTAT6800.035.965758-59
CGGAAGA13900.035.8757444
CAGTACG8000.033.84557734-35
GGTCGCC7500.033.55587454-55
TGGTCGC7450.032.5063254-55
AGCGTCG7650.032.2875310-11
AAGAGCG15450.032.276117
GCGTCGT7700.031.76943210-11
TCGGACG1201.5095611E-731.6613253
CGTCGTG8200.030.99166912-13
AGTACGT8550.030.83328236-37
Produced by FastQC (version 0.11.5)