FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314441

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314441
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8682687
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG10500.026.6888648-49
AATGCGC12550.023.84235634-35
GATCGGA25250.023.5613271
CTCGTAT11700.023.3427444-45
GCGCATC11650.023.03435738-39
TAATGCG12700.022.9997834-35
ATCGGAA26600.022.5322462
CGTATGC12550.021.95086346-47
TCTCGTA12300.021.81759642-43
ATGCGCA13750.021.41614736-37
TGCGCAT13550.021.20647236-37
TCGGAAG27950.021.0714653
TCGTATG13850.020.4050244-45
CGGAAGA29200.020.1696644
CGCATCT14450.019.5570238-39
ATGCCGT14600.019.51936748-49
ATCTCGT15950.016.52707142-43
GCCGTCT15450.016.4473250-51
CGTTGGA35550.016.33321
TGCCGTC15450.016.29360850-51
Produced by FastQC (version 0.11.5)