FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314442

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314442
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8682687
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA7000.037.65263758-59
TCGCCGT7750.033.08881856-57
GTCGCCG8900.029.34687656-57
GAGCGTC19900.025.5394299
GCCGTAT11050.024.49700758-59
GTACGTC10950.024.0752430-31
GATCGGA24700.022.4656851
CCGTATC12450.022.3148160-61
CGTATCA12500.022.0355960-61
ATCGGAA25150.021.5274352
AGAGCGT24300.021.4997988
TGGTCGC12350.021.33997754-55
TACGTCC12950.020.72522232-33
GGTCGCC13000.020.45561654-55
GTGTACG13500.020.22778328-29
TCGGAAG27900.019.575333
GTGGTCG13800.019.09778852-53
AAGAGCG29950.018.7112647
GCGTCGT14150.018.4639910-11
CGTCGTG14250.018.33515512-13
Produced by FastQC (version 0.11.5)