FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314445

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314445
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4081233
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG6200.029.11005648-49
GATCGGA13500.028.2036191
GCGCATC6150.027.80164738-39
TCGGAAG14300.027.233933
CTCGTAT6500.027.03631644-45
ATCGGAA14500.026.2430762
TAATGCG6950.025.62651834-35
TCTCGTA6650.025.35481642-43
AATGCGC7250.025.22120934-35
CGTATGC7450.024.22626546-47
CGGAAGA15700.023.8990824
TGCGCAT7650.023.5920336-37
ATGCGCA7650.023.28160936-37
TCGTATG8050.021.83056444-45
CGCATCT8150.020.6877838-39
ATCTCGT8700.020.4722642-43
ATGCCGT8700.019.92621248-49
TGCCGTC7850.019.05852750-51
GCCGTCT8250.017.8466350-51
CATCTCG9900.017.75035340-41
Produced by FastQC (version 0.11.5)