FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314448

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314448
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6389817
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCT87120.13634193279713644Illumina Single End PCR Primer 1 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTC21500.043.077219
CGCCGTA11550.041.93872558-59
GATCGGA24350.039.425631
GTCGCCG12650.038.47883256-57
TCGCCGT12050.037.43907556-57
ATCGGAA24900.037.381542
AGAGCGT24500.037.217968
TCGGAAG26650.035.460883
TGGTCGC14250.033.65746754-55
CGGAAGA27700.033.430934
GCCGTAT13950.033.36175558-59
GGTCGCC14300.033.0416754-55
CCGTATC14550.032.96556560-61
AAGAGCG29450.032.4118927
CGTATCA16150.029.55259760-61
TCGGACG1802.1282176E-1029.0210973
GTGGTCG16450.028.14580352-53
AGCGTCG17200.027.48004310-11
CGGCGTA1056.918941E-527.1380988
GCGTCGT16850.027.06413510-11
Produced by FastQC (version 0.11.5)