FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314449

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314449
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6265364
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAA5900.035.41975836-37
TCCGCGA6000.035.2252234-35
CCGCGAA6100.034.64775534-35
TATGCCG6750.028.8497748-49
CTCCGCG8150.025.93257332-33
GATCGGA18100.024.7207871
CGTATGC8300.024.03460346-47
ATCGGAA19100.023.659452
TCGGAAG19200.023.0050533
CTCGTAT8600.022.9200444-45
GCGAAAT8600.022.64281336-37
TCGTATG8800.022.39913244-45
TCTCGTA8500.022.07167242-43
ACTCCGC9700.021.78870632-33
CGGAAGA21550.020.4963824
ATGCCGT9800.019.38635648-49
TGCCGTC9450.019.3506150-51
CACTCCG11900.018.76214630-31
ATCTCGT10400.017.81142-43
ACACGTC13800.016.34760912-13
Produced by FastQC (version 0.11.5)