FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314451

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314451
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4253577
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT62610.14719376186207514TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA18400.038.8038331
ATCGGAA18900.037.7501452
TCGGAAG19250.037.255173
TATGCCG9400.034.8645948-49
CGGAAGA21350.033.3686644
CGTATGC9850.033.27081346-47
CTCGTAT10450.031.58852244-45
TACTCGA11350.030.75650236-37
TCTCGTA10150.030.65003442-43
ATGCCGT11000.030.44106148-49
TTACTCG11650.029.76064934-35
TCGTATG11500.029.32387244-45
GCCGTCT10600.028.2301650-51
TGCCGTC10600.028.2301650-51
ACTCGAT12800.025.41710336-37
ATCTCGT12200.025.30516642-43
CGTCTTC11250.024.06597952-53
CGTCTGA15450.022.74855416-17
CCGTCTT12350.021.9224552-53
ACACGTC16700.021.33009512-13
Produced by FastQC (version 0.11.5)