FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314452

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314452
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4253577
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCT58460.1374372675045027Illumina Single End PCR Primer 1 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTC13700.042.6461269
CGCCGTA7400.042.3545458-59
GTCGCCG7850.039.92469456-57
TCGCCGT7950.038.82518856-57
GATCGGA16850.037.231091
AGAGCGT16200.036.9419568
GCCGTAT8900.035.4829258-59
ATCGGAA18550.034.8187682
TCGGAAG19000.034.244073
AAGAGCG18200.033.92237
TGGTCGC9050.033.58001354-55
CCGTATC9600.033.1437460-61
GTGGTCG9450.032.91332252-53
GGTCGCC9700.032.7983954-55
CGGAAGA19250.032.319084
TCGTACG600.00395549331.6611843
CGTATCA10900.029.40865960-61
TCTCGGT11250.028.07803548-49
GCGTCGT10950.027.33174710-11
CGTCGTG11200.026.93500912-13
Produced by FastQC (version 0.11.5)