FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314453

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314453
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6396593
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGCA2650.029.5711936-37
CGCGCAT2600.026.4865536-37
TCTCGCG3350.025.52198634-35
CTCGCGC4350.018.01695634-35
TATGCCG4700.017.68340148-49
GATCGGA8900.017.163861
CTCGTAT4750.016.49748244-45
ATCGGAA10450.015.4745042
AACGGGA21350.015.34816558
GCGCATC5000.015.19839438-39
TCGGAAG10950.015.1843093
ACGGGAT15650.014.5657269
CGTTGGA23750.014.2708471
AAACGGG26350.014.0578617
ACTCTCG7350.013.24871232-33
CCCGAAC4102.4978326E-612.8074851
CGTATGC6400.012.61511446-47
TCGTATG6300.012.43857744-45
CGGAAGA13100.012.3278574
TCTCGTA5550.011.98048142-43
Produced by FastQC (version 0.11.5)