FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314455

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314455
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6695235
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACGC2250.033.822122
CACGACG2600.029.3832041
CGACGCT3150.025.7686021
TATGCCG6750.024.27406348-49
CGTATGC7700.020.97050546-47
CTCGTAT7700.020.66165244-45
TCTCGTA7850.019.66244942-43
TCGTATC8450.018.8285538-39
GATCGGA17500.018.5533941
GTCACGA9550.017.90622928-29
ATTCGTA9200.017.80930936-37
TCGTATG9400.017.17758244-45
TCGGAAG19300.016.984343
ATGCCGT10150.016.84471548-49
CGGAAGA19900.016.469794
ATCGGAA19450.016.383952
TATCTCG9450.016.33341840-41
GCCGTCT10200.016.06371950-51
ACGAATT11000.015.545979532-33
TGCCGTC10850.015.32023750-51
Produced by FastQC (version 0.11.5)