FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314458

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314458
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4991995
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGTAGATCT62530.12526054212794685Illumina Single End PCR Primer 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTC16300.048.0697829
GATCGGA18900.043.1078341
AGAGCGT19100.041.7529458
CGCCGTA9500.041.48261658-59
ATCGGAA20100.041.069872
TCGGAAG20350.040.5612643
GTCGCCG10350.038.30190356-57
TCGCCGT10550.038.23890356-57
AAGAGCG21450.037.3964087
CGGAAGA21950.037.179724
GCCGTAT11300.036.31928658-59
GTACGTC11150.035.7661530-31
GGTCGCC11800.034.7822554-55
TGGTCGC11500.034.06735654-55
CCGTATC11850.033.25677560-61
TACGTCC12100.031.99437732-33
GTGGTCG13050.031.81040252-53
CGTATCA13000.031.7498560-61
CGGCGTA753.429798E-431.0908878
GTGTACG12750.030.91140628-29
Produced by FastQC (version 0.11.5)