FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314460

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314460
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5281420
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA4650.039.61660458-59
GATCGGA9900.039.2479741
ATCGGAA10800.035.8446582
GAGCGTC10900.034.655749
GTCGCCG5600.033.31296556-57
TCGCCGT5950.031.35337856-57
TCGGAAG12900.030.7288193
TGGTCGC6600.028.2660854-55
AGAGCGT13400.027.8410368
GCGTCGT6950.026.84044610-11
AAGAGCG14450.026.4634347
CGGAAGA14600.025.8731214
CGACGCT2208.0035534E-1125.8462261
GCCGTAT7150.025.76464558-59
GGTCGCC7250.025.41023354-55
GTGGTCG7900.025.09216352-53
CGTCGTG7450.024.72585312-13
AGCGTCG7650.024.68926610-11
CCGTATC8100.022.74286560-61
CGTATCA8550.021.27313860-61
Produced by FastQC (version 0.11.5)