FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314463

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314463
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5712729
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT68970.12073038997648936TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA15000.050.910511
ATCGGAA16850.046.00682
TCGGAAG18550.041.741523
CGGAAGA21350.036.26314
TATGCCG11250.034.82850348-49
CTCGTAT11500.033.24582744-45
CGTATGC12300.031.4692146-47
TCTCGTA11700.030.24232342-43
TCGTATG13400.028.7090844-45
ACGACGC2201.8189894E-1228.1031512
ATCTCGT13900.026.3099942-43
ATGCCGT14400.026.05541248-49
TGCCGTC14650.025.9347450-51
ACTCCGG16200.024.63041732-33
GCCGTCT15000.024.53804850-51
CACGACG2751.8189894E-1224.2981971
CCGGAGA15750.024.27738434-35
CACTCCG16350.023.96876130-31
CGACGCT2801.8189894E-1223.8643021
CGGAGAA16950.023.25646236-37
Produced by FastQC (version 0.11.5)