FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314466

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314466
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5059746
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGCG1452.1827873E-1135.3799676
CGGCATA4100.034.1247988
TACGCCG753.4294176E-431.0914866
CGCCGTA3550.029.55953658-59
TCGCCGT4000.027.4008656-57
TGATACG5750.021.8990043
GTCGCCG4700.021.83137956-57
ATACGGC4250.018.6543395
TGGTCGC5900.018.18187154-55
GGTCGCC6100.017.96804454-55
GTGGTCG6400.017.85525352-53
GCCGTAT6350.017.62710458-59
ACGGCAT6100.017.584537
TCGCGGA1255.438551E-616.78923622-23
GATACGG5100.016.4600354
CCGTATC6950.015.76926660-61
ACGGCGA2407.197219E-515.5457437
CGTATCA6800.015.43128360-61
CCGTGTA7500.015.20929838-39
GACGGCA6800.015.0885156
Produced by FastQC (version 0.11.5)