FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314468

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314468
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5253726
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA8200.041.6987258-59
GAGCGTC16150.040.880549
GTCGCCG9800.035.6176556-57
GATCGGA20100.035.2824441
TCGCCGT9900.033.57893456-57
AGAGCGT19700.033.5121428
TCGGAAG21150.033.0064353
ATCGGAA20950.032.8681762
GCCGTAT10250.032.66399458-59
TGGTCGC10900.031.58616654-55
CGGAAGA22150.031.3025024
CAGTACG11150.030.4620634-35
GGTCGCC11250.030.39243154-55
AAGAGCG22900.029.6563017
AGTACGT12000.028.6980136-37
CGTATCA12150.028.33777660-61
CCGTATC12650.028.15624860-61
GTGGTCG12100.028.06052652-53
GCGTCGT12750.025.70657710-11
AGCGTCG13250.025.63276910-11
Produced by FastQC (version 0.11.5)