FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314470

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314470
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4641968
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAGATCT160090.34487527703767024Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTC41050.048.854459
AGAGCGT44000.046.426968
AAGAGCG46950.043.807847
GATCGGA49250.043.0028231
CGCCGTA24200.042.4956458-59
GTCGCCG24500.041.785656-57
TCGGAAG49450.041.7808153
CGGAAGA49800.041.3930784
ATCGGAA49700.041.3874322
AGTACGT27900.041.0187836-37
GCCGTAT25600.040.35384458-59
TCGCCGT25100.040.32219756-57
TGGTCGC25150.040.05723654-55
TCGGACG1750.039.9753883
GGTCGCC25900.039.61759654-55
ACGTGTA26300.039.61398338-39
CGTATCA26600.039.09957560-61
CCGTATC26550.038.7340560-61
TACGGCG854.205831E-738.408556
GTGGTCG26500.038.28223452-53
Produced by FastQC (version 0.11.5)