FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314471

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314471
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4139960
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCGAA4550.038.62281434-35
CGCGAAA4500.037.4687736-37
TCCGCGA4750.035.99667734-35
GATCGGA11150.032.0111351
TATGCCG5400.030.34479348-49
ATCGGAA11850.030.1052442
TCGGAAG12100.029.4387233
CTCCGCG6700.026.58321232-33
CGTATGC6450.025.77328546-47
CGGAAGA13900.025.6268234
CTCGTAT7000.023.7486744-45
TCGTATG7300.023.7486744-45
GCGAAAT6600.023.74781236-37
ACTCCGC7750.022.98161532-33
ATGCCGT7150.022.91774648-49
TCTCGTA7050.021.55881742-43
TGCCGTC7250.019.9815250-51
ACACGTC10000.018.99767512-13
CACTCCG9550.018.90140530-31
ATCTCGT8150.018.35764742-43
Produced by FastQC (version 0.11.5)