FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314472

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314472
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4139960
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA3850.038.24117758-59
GAGCGTC8900.033.617279
GATCGGA9900.031.6633051
TCGCCGT4450.031.48322356-57
GTCGCCG5200.030.5955856-57
ATCGGAA10200.029.79582
TCGGAAG11500.028.0793023
GCCGTAT5300.027.33092158-59
AGAGCGT10850.027.137758
GCGTCGT5550.026.10074410-11
CGGAAGA12350.025.7625164
TGGTCGC6250.025.45459454-55
CGGCATA2551.8189894E-1124.2111328
CCGTATC6400.024.1181660-61
AGCGTCG6150.023.94046610-11
CGTCGTG6050.023.55213412-13
CGTATCA6400.023.00501460-61
GTGGTCG6750.022.16169452-53
AAGAGCG14200.022.071987
GGTCGCC7250.021.28858454-55
Produced by FastQC (version 0.11.5)