FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314473

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314473
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6248301
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCAT4800.029.68424436-37
TCTCGCG5000.028.97182334-35
TCGCGCA4900.028.59380136-37
CTCGCGC5950.025.144334-35
ATCGGAA13250.022.615732
GATCGGA13050.022.2474541
TATGCCG6200.021.8327248-49
GCGCATC6900.020.30582438-39
CTCGTAT6950.019.81844544-45
CGTATGC7300.018.86794546-47
TCGGAAG15800.018.636453
TCTCGTA7450.018.1692242-43
TCGTATG8400.017.24554644-45
CGCATCT8300.017.1668638-39
CGGAAGA17450.017.1471164
CGTTGGA24400.014.824661
ACTCTCG9850.014.70621532-33
ATGCCGT9500.014.24872348-49
ATCTCGT10250.013.43760442-43
CTCTCGC11700.013.39569932-33
Produced by FastQC (version 0.11.5)