FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314479

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314479
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4368835
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG6800.031.77950748-49
GATCGGA14700.031.0855791
ATCGGAA15400.029.651852
CGGCTAT7450.029.3252234-35
CGGAAGA15650.029.1347664
TCGGAAG16200.028.144013
TCGTATG8600.025.68054244-45
CGTATGC8350.025.59577446-47
CTCGTAT8100.025.50667844-45
ATGCCGT8550.025.2749348-49
TCTCGTA7800.024.05191642-43
TGCCGTC8400.024.03030650-51
ACCGGCT9150.023.87668232-33
TCACCGG10050.022.45337530-31
CACCGGC10050.022.45337530-31
GCCGTCT9150.022.0606150-51
ATCTCGT8750.021.44056542-43
GTCACCG10850.020.79818228-29
CGTCTTC9850.019.76957352-53
CCGTCTT10350.018.58507552-53
Produced by FastQC (version 0.11.5)