FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314481

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314481
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5424956
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT93490.17233319496047525TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG14550.036.88672348-49
CTCGTAT15350.034.34592444-45
CGTATGC15700.034.1848346-47
TCTCGTA14800.033.53568642-43
ATGCCGT15650.033.2318448-49
TCGTATG16400.032.2917544-45
CGGCTAT16700.032.27839734-35
TGCCGTC14750.031.39635550-51
ATCTCGT16200.031.22390442-43
CGGAAGA37550.029.7236774
TCGGAAG37950.029.5344453
ATCGGAA38450.029.4427382
GCCGTCT15050.029.19253750-51
GATCGGA39100.028.7282731
TCACCGG20400.027.24522430-31
ACCGGCT20950.026.18331132-33
GTCACCG21500.025.18889228-29
CGTCTTC16650.024.67553152-53
CCGTCTT17300.024.57205852-53
CACCGGC22750.024.01325830-31
Produced by FastQC (version 0.11.5)