FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314484

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314484
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5565891
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA4650.036.76706758-59
GTCGCCG5550.031.65996656-57
TCGCCGT5200.031.50775156-57
TGGTCGC6050.029.04173954-55
GAGCGTC12800.027.8287669
GGTCGCC6750.026.03000554-55
GCCGTAT6800.025.8405858-59
AGAGCGT15800.023.7457188
GATCGGA14700.023.6355291
TCGGAAG16550.022.0955163
CGGAAGA17050.022.0046314
ATCGGAA15800.021.9418532
AAGAGCG16650.021.6771897
CCGTATC8200.021.42906260-61
CGTATCA8600.020.98458960-61
GTGGTCG8450.020.79338652-53
CGGCATA3201.8189894E-1120.7775048
CCGTGTA8750.020.08137338-39
TCTCGGT9900.019.19078848-49
GAGCCGT9500.018.99811236-37
Produced by FastQC (version 0.11.5)