FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314487

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314487
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5308899
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG7050.030.9908348-49
CGTATGC8000.028.79548646-47
CTCGTAT8150.028.55744444-45
TCTCGTA7900.026.75466342-43
TCGTATG9350.023.87630744-45
GATCGGA21950.021.6796861
ATGCCGT10050.021.50353448-49
ATCGGAA22900.020.9770262
ATCTCGT10300.020.75113342-43
ACTCCGG11450.020.53201132-33
CACTCCG11350.020.50755130-31
CGGAAGA23900.020.070934
TCGGAAG23800.019.7559623
CCGGAGA12100.019.23297934-35
TGCCGTC10500.018.320250-51
CGGAGAA13350.017.25426136-37
CTCCGGA13200.016.91047132-33
TCCGGAG13950.016.68237134-35
CGTCTGA14250.016.49796316-17
GCCGTCT11000.015.97620250-51
Produced by FastQC (version 0.11.5)