FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314490

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314490
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3977679
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGGC2800.040.706635
CGCCGTA3750.036.09502458-59
CGGCATA3000.031.6638988
TACGGCA3400.030.7295156
TCGCCGT4550.030.26960256-57
GCCGTAT4850.029.86701858-59
GATACGG3950.027.6530284
GTCGCCG4700.027.28261856-57
TGATACG4700.027.2821033
GGTCGCC5550.024.38592554-55
CGTATCA5900.024.14936860-61
GAGCGTC11250.024.0651659
TGGTCGC5850.021.91771354-55
CCGTATC6350.021.31609360-61
GTGGTCG6750.020.0500252-53
AGAGCGT13100.019.578448
ACGGCAT4650.019.4064167
GGCGTAC1559.634226E-418.3859529
GATCGGA16600.017.4401931
AAGAGCG15800.017.4347467
Produced by FastQC (version 0.11.5)