FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314491

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314491
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4175693
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG6950.030.41052848-49
CTCGTAT7150.029.55988344-45
CGTATGC7450.028.36972246-47
TTACTCG7650.027.9377934-35
TACTCGA7750.027.88371536-37
TCTCGTA7200.027.04565442-43
ATGCCGT8300.024.89200848-49
ATCTCGT8550.023.88627842-43
TCGTATG8650.023.88481744-45
TGCCGTC8150.022.7278550-51
ACTCGAT9550.021.38484236-37
GCCGTCT8550.020.83130650-51
CGTTGGA15050.017.3883481
CGTCTTC9450.017.33937552-53
CGGAAGA26150.016.8908794
TCGGAAG26200.016.8586433
CCGTCTT10950.016.26534752-53
ATCGGAA27250.016.2327562
GATCGGA27750.016.1174761
AAACGGG17800.014.946847
Produced by FastQC (version 0.11.5)