FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314493

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314493
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3107248
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA5550.024.0880831
CTCGTAT3000.022.1635144-45
ATCGGAA6350.021.7184832
CGAAACG1553.915417E-521.4471765
ACGACGC1150.0036993520.6765162
CGTATGC3400.020.2541446-47
TATGCCG3450.019.96092448-49
CACGACG1200.004624979619.8941761
TCTCGTA3050.018.68616342-43
CGGAAGA7100.018.062264
TCGGAAG7400.017.9730153
ATGCCGT4100.017.37557248-49
AACGGGT3803.927198E-916.246648
TCGTATG4250.016.20357344-45
TGCCGTC4250.015.64457850-51
AAACGGG13800.015.1417967
CGTTGGA12300.015.1389841
TCCGATC2205.7045807E-415.1136723
GCCGTCT4800.014.34668350-51
GAAACGG14750.014.1665616
Produced by FastQC (version 0.11.5)