FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314496

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314496
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7257077
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA3100.024.07117758-59
TCGCCGT3600.023.3187956-57
GTCGCCG4250.019.2037156-57
GGTCGCC6500.015.78519254-55
GCCGTAT5900.014.6237558-59
CGTTGGA27600.013.7298771
TGGTCGC6250.013.05865854-55
GAGCGTC11950.012.8786439
GTGGTCG7450.012.52093452-53
CCGTATC6800.011.65955560-61
GATCGGA14000.011.5036761
GCGTCGT7100.011.49482310-11
ATCGGAA15000.010.57235052
CGTATCA8400.010.54912360-61
AACGGGA28000.010.492958
AAACGGG34250.010.4845127
TCGGAAG16250.010.331523
CGTCGTG7750.010.229897512-13
AGAGCGT15550.09.8968888
GAAACGG35300.09.7763146
Produced by FastQC (version 0.11.5)