FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314497

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314497
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5312560
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT8700.030.2992744-45
CGTATGC9400.028.54768446-47
TATGCCG9100.028.4444348-49
GCGATAG10250.028.03246734-35
CGATAGA9750.028.00870736-37
TCTCGTA8200.027.51275842-43
AGCGATA10700.026.63160334-35
TCGTATG10300.025.5925944-45
GATCGGA22250.025.0318181
TCGGAAG22450.024.9626923
ATCGGAA22450.024.7922122
ATGCCGT11100.023.53324348-49
CGGAAGA23650.023.4959354
TGCCGTC10900.021.5690550-51
CAGCGAT13800.020.82112132-33
ATCTCGT10400.020.77937342-43
ACAGCGA14100.020.20970332-33
GCCGTCT12600.018.65894150-51
CGTCTGA16600.017.30920616-17
ACACGTC16950.017.09228712-13
Produced by FastQC (version 0.11.5)