FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314499

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314499
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2976167
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT30670.10305201287427757TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA7800.036.7309461
ATCGGAA8500.033.5739062
TCGGAAG8700.032.21693
CGGAAGA9050.030.9667784
TATGCCG4700.030.81921848-49
CTCGTAT4750.029.49522644-45
TCTCGTA4300.027.61250142-43
CGTATGC5150.027.20410546-47
ACGACGC700.00833955827.1788732
TCGTATG6050.023.94240444-45
TGCCGTC5950.022.74822650-51
ATGCCGT6250.022.03624548-49
CCGGAGA6800.022.002734-35
CACTCCG6600.021.95181330-31
GCCGTCT5950.021.95004350-51
CGTCTGA7150.020.92265716-17
ACTCCGG7150.020.5950532-33
ATCTCGT6000.020.5805242-43
CGGAGAA7400.019.57453236-37
ACACGTC7500.018.99612612-13
Produced by FastQC (version 0.11.5)