FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314500

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314500
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2976167
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTC6550.039.1585279
CGCCGTA3600.036.2704758-59
GTCGCCG3800.034.9742256-57
AGAGCGT7400.034.6594248
GATCGGA7500.033.480881
TCGGAAG7800.033.479313
ATCGGAA7900.033.0588572
TCGCCGT4100.031.27775656-57
GCGTCGT4350.030.01744710-11
CGGAAGA9200.029.398374
GCCGTAT4600.027.87870258-59
CCGTGTA5050.027.51411638-39
TGGTCGC4750.027.48919954-55
CGTCGTG4650.027.07749412-13
AGCGTCG4900.026.6481410-11
GGTCGCC5100.026.51706954-55
AAGAGCG9800.025.6955597
GAGCCGT6300.024.04183636-37
TCGTGTA5450.023.95767214-15
CCGTATC5650.023.93616360-61
Produced by FastQC (version 0.11.5)