FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005314501

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005314501
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4844555
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT74140.1530377919127763TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA16300.055.362921
ATCGGAA18100.050.454362
TCGGAAG19100.047.2590333
ACGACGC2250.046.5067752
CACGACG2300.045.6709061
CGGAAGA20100.044.9013444
TATGCCG11800.037.6318248-49
CTCGTAT11900.036.7163744-45
CGTATGC13250.033.51327546-47
CGACGCT3150.033.3470081
TCTCGTA12200.033.08976442-43
TCGTATG13650.032.70499444-45
TGCCGTC13800.031.83378450-51
ATGCCGT14300.030.72071648-49
CCGGAGA14800.030.4869834-35
CACTCCG15600.029.53398530-31
GCCGTCT14450.029.4158150-51
ACTCCGG15750.028.64911832-33
CGGAGAA16050.027.81452836-37
CGTCTGA16800.027.56165316-17
Produced by FastQC (version 0.11.5)